Section outline

Main course page

  • Learn the essential steps for collecting and handling samples during a suspected lumpy skin disease (LSD) outbreak in this short video. Discover which specimens to collect, how to ensure proper transport, and the key diagnostic tests for laboratory confirmation.

    This session supports veterinarians in achieving accurate and reliable LSD diagnosis.
    •   Here are the key facts we learnt in this module:

      • Accurate diagnosis of LSD relies on collecting critical samples
      • Skin nodules, saliva swabs and blood with EDTA are useful for virus detection by PCR and virus isolation, in early stages of the disease. 
      • Blood without anticoagulant (serum samples) for detection of antibodies could be collected but bear in mind that antibodies do not appear till at least two weeks after infection.
      • PCR is the gold standard for detecting LSD viral DNA in skin and mucosal samples, providing a rapid and sensitive diagnostic tool.
      • Virus isolation from blood samples can confirm active infection, while antibody detection in serum helps identify animals with past exposure or vaccination history.

      Please refer also this additional resource:


      In the next session we will focus on identification of lesions.